Recombinant Mouse IL-22重组小鼠白介素22

Description :
FAQ COA 已发表文献 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147aa mature protein that shares approximately 79% and 22%aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family. Product Properties Synonyms IL-TIF, IL-TIF alpha, IL-22a Accession Q9JJY9 GeneID 50929 Source E.coli-derivedMouse IL-22, Leu34-Val179. Molecular Weight Approximately 16.6 kDa. AA Sequence LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK KLGESGEIKA IGELDLLFMS LRNACV Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96% by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 106IU/mg. Fully biologically active when compared to standard. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 1 PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.