Recombinant Human Proinsulin C-Peptide Analogue

Description :
FAQ COA 已发表文献 This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5′ region and with the IGF2 gene at the 3′ region. Alternative splicing results in multiple transcript variants. Product Properties Synonyms Human Proinsulin C-Peptide Analogue, IDDM Protein, Human; IDDM1 Protein, Human Accession P01308 GeneID 3630 Source E.coli-derived human Proinsulin C-Peptide Analogue protein,Arg55-Arg89 Molecular Weight Approximately 3.6 kDa. AA Sequence RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95 % by SDS-PAGE and HPLC analyses. Biological Activity Data Not Available. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.