Recombinant Human MCP-1/CCL2 重组人MCP-1/CCL2

Description :
FAQ COA 已发表文献 CCL2, also called monocyte chemotactic protein1 (MCP1) or JE, is a member of the CC or beta chemokine family that is best known as a chemotactic agent for mononuclear cells .Human CCL2 cDNA encodes a 99amino acid (aa) precursor protein with a 23aa signal peptide and a 76aa mature protein . Removal of the first5aa of the mature protein, including the N-terminal pyrrolidone carboxylic acidmodified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding. CCL2 may form multiple bands from 8.713.5 kDa on SDSPAGE due to noncovalent dimerization and variable carbohydrate content. Mature human CCL2 shares 7879% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49aa and shares only 56%aa identity within the common region. Human CCL2 can, however, induce a response in murine cells. Product Properties Synonyms HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2 Accession P13500 GeneID 6347 Source E.coli-derived Human CCL2 ,Gln23-Thr99. Molecular Weight Approximately 8.7 kDa AA Sequence QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL. Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.