Recombinant Mouse Fc gamma RIIB/CD32b Protein,His Tag

Description :
Source Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-terminal. It contains Thr40-Arg217.[Uniprot|NP_001070657.1] Molecular Weight The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity SPR Data: OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 M as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.