Recombinant Human Amphiregulin

Description :
FAQ COA 已发表文献 Amphiregulin is an EGF related growth factor that signals through the EGF/TGF- receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulins extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues. Product Properties Synonyms AR, AREG, Colorectum Cell-derived Growth Factor, CRDGF Accession P15514 GeneID 374 Source E.coli-derived Human Amphiregulin protein,Val60-Leu108. Molecular Weight Approximately 11.3 kDa. AA Sequence SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/mL. Endotoxin Formulation Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.