Recombinant Biotinylated Human Fc gamma RIIA/CD32a(R167) Protein,His-Avi Tag

Description :
Source Recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(R167).[Uniprot|P12318-1] Molecular Weight The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data:Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.17 M as determined in SPR assay. SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag with an affinity constant of 1.03 M as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.