Recombinant Human CCL20/MIP-3 alpha Protein 重组人MIP-3α/CCL20

Description :
FAQ COA 已发表文献 CCL20, also known as LARC (Liver and Activation-regulated Chemokine) or MIP3A(Macrophage Inflammatory Protein-3),is a small cytokine belonging to the CC chemokine family.CCL20is Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10.CCL20has been shown to be chemotactic for lymphocytes. This chemokine may Inhibit proliferation of myeloid progenitors in colony formation assays and it may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. Chemokine CCL2 was shown to play a role in colorectal cancer (CRC) pathogenesis. Product Properties Synonyms LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine Accession P78556 GeneID 6364 Source E.coli-derived Human MIP-3/CCL20, Ala27-Met96. Molecular Weight Approximately 8.0kDa. AA Sequence ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/mL.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.