Recombinant Human I-309/CCL1重组人 I-309/CCL1

Description :
FAQ COA 已发表文献 CCL1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma / T cell line but not in EBV-transformed B cells.CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein.CCL1 interacts with the G protein-linked transmembrane chemokine receptors CCR8 and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that CCL1 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, CCL1was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. CCL1 induces significant levels of LTC4 from elicited eosinophils. Product Properties Synonyms Small-inducible cytokine A1, T lymphocyte-secreted protein I-309 Accession P22362 GeneID 6346 Source E.coli-derived Human I-309/CCL1, Ser23-Lys96. Molecular Weight Approximately 8.6kDa. AA Sequence SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.