Recombinant Human IL-36γ,169aa

Description :
FAQ COA 已发表文献 IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL1 beta, IL1 alpha, IL1ra, IL18, IL36Ra (IL1F5), IL-36 alpha (IL1F6), IL36 beta (IL1F8), IL37 (IL1F7) and IL-1F10. All family members show a 12 beta strand, beta trefoil configuration, and are believed to have arisen from a common ancestral gene. IL36 gamma is an 1822 kDa, 169 amino acid intracellular and secreted protein that contains no signal sequence, no prosegment and no potential Nlinked glycosylation sites. Human IL36 gamma (aa18169) shares 58%, 59%, 68% and 69%aa sequence identity with mouse, rat, bovine and equine IL36 gamma, respectively, and 2357%aa sequence identity with other family members. 134aa isoform missing aa1953 has been reported. Highest levels of IL36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to firstline defense against pathogens in the skin, lungs and digestive tract. Its expression is induced by LPS treatment of monocytes, and by IL alpha / beta, IL17 or TNF alpha treatment of keratinocytes and bronchial epithelia. Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis. It is elevated in inflammatory disordersofthelung (such as asthma) and viral infections. Lung IL36 gamma and other IL36 proteins contribute to neutrophil influx. The receptor for IL-36 gamma is a combinationof IL1Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL1RAcP. IL36 alpha,betaand gamma all activate NF kappa B and MAPK pathways in an IL1Rrp2 dependent manner, and IL36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL8. Product Properties Synonyms IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1 Accession Q9NZH8 GeneID 56300 Source E.coli-derived human Interleukin-36 gammaprotein, Met1-Asp169, with an N-terminal Met. Molecular Weight Approximately 18.7 kDa. AA Sequence MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36 at 1 g/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 g/mL. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.