Recombinant Human IL-36α,153aa

Description :
FAQ COA 已发表文献 Human IL-36 alpha, previously called IL-1F6and FIL1 epsilon (family of IL-1 member epsilon), is amember of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL38 (IL1F10) . All family members show a 12 beta strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene. IL-36 alpha is an 1822kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from Nlinked glycosylation sites. It can bereleased in response to LPS and the cellATPinduced activation of the P2X7 receptor. A 120aa isoform missing aa 138 has been reported. Human IL36 alpha (aa6 158) shares 5768% aa sequence identity with mouse, rabbit, equine and bovine IL36 alpha and 2757%aa sequence identity with other novel IL1 family members. IL36 alpha is mainly found in skin and lymphoid tissues,butalsoin fetal brain, trachea, stomach and intestine . It is expressed by monocytes, Band Tcells. The receptor for IL36 alpha is a combination of IL1Rrp2 (also called IL1RL2 or IL1R6), mainly found in epithelia and keratinocytes, and the widely expressed IL1RAcP. IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL1Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8. IL-36 alpha andotherfamily members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL36 alpha in skin keratinocytes produces epidermal hyperplasia. IL-36 alpha is present in kidney tubule epithelia, andit is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis. Product Properties Synonyms FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1 Accession Q9UHA7 GeneID 27179 Source E.coli-derived human Interleukin-36 alphaprotein, Lys6-Phe158. Molecular Weight Approximately 17.1 kDa. AA Sequence KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 105IU/mg. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1% Tween 80. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.