Recombinant Human Galectin-1 Protein

Description :
FAQ COA 已发表文献 Galectin-1, a polypeptidic factor that can have major effects on cell growth and apoptosis, is an important regulator of leukocyte function and tumor angiogenesis. Galectin-1 belongs to a family of 15 structurally related -galactoside binding proteins that are able to control a variety of cellular events, including cell cycle regulation, adhesion, proliferation, and apoptosis. Human Galectin-1 shares 88% aa sequence identity with mouse, equine and ovine, 90% with rat, and 87% with bovine and porcine Galectin-1.Recombinant human galectin-1 to three porphyrin compounds: Zn-porphyrin (ZnTPPS). Mn-porphyrin and Au-porphyrin. These compounds are widely applied in the photodynamic therapy of cancer. Galectin-1 can also modulate cell-cell and cell-matrix interactions. Product Properties Synonyms Beta-galactoside-binding lectin L-14-I,BHL,DKFZp686E23103,GAL1,gal-1,Galaptin,galectin 1,Galectin1,Galectin-1,GBP,HBL,HLBP14,HPL,L-14,Lactose-binding lectin 1,LGALS1 Accession P09382 GeneID 3956 Source E.coli-derived HumanGalectin-1, Ala2-Asp135. Molecular Weight Approximately 14.6kDa. AA Sequence ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95%by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/mL.Fully biologically active when compared to standard. Endotoxin 0.1EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4, with 1 mM DTT. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.