Recombinant Mouse FGF-8 Protein

Description :
FAQ COA 已发表文献 FGF-8 is a member of the fibroblast growth factor (FGF) family that was initially identified as an androgen-inducible growth factor in a mammary carcinoma cell line.FGF-8 is one of the key signaling molecules implicated in the initiation, outgrowth, and patterning of vertebrate limbs.The cloned 1.26-kb cDNA contained an open reading frame encoding 212 amino acid residues with 84%, 86%, and 80% amino acid identities to those of Xenopus, chick, and mouse, respectively. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis. FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells. Product Properties Synonyms AIGF,AIGFKAL6, HBGF-8, FGF-8c Accession P37237 GeneID 14179 Source E.coli-derived mouse FGF-8, Gln23-Arg268. Molecular Weight Approximately 28.1 kDa. AA Sequence QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97%by SDS-PAGEand HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/mL, corresponding to a specific activity of > 2.0 105IU/mg in the presence of 10 g/ml of heparin. Fully biologically active when compared to standard. Endotoxin 1.0EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4, 500 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.