Recombinant Mouse bFGF/FGF-2 Protein重组小鼠碱性成纤维细胞生长因子

Description :
FAQ COA 已发表文献 FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF superfamily of mitogenic proteins which show 35-60%amino acid conservation.Human FGF acidic shares54%aminoacid(aa)sequenceidentitywithFGFbasicand 17%-_x005f_x001f_33%withotherhumanFGFs.Itshares92%,96%,96%,and96%aasequenceidentitywithbovine,mouse,porcine,andratFGFacidic,respectively,andexhibitsconsiderablespeciescrossreactivity.AlternatesplicinggeneratesatruncatedisoformofhumanFGFacidicthatconsistsoftheN_x005f_x001f_terminal40%ofthemoleculeandfunctionsasareceptorantagonist. The effects of androgen and FGF-2 could be partly reversed with a specific anti-FGF-2 immunoglobulin G or by suramin, which inhibits binding of FGFs to their high affinity receptors. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems. Product Properties Synonyms bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin Accession P15655 GeneID 14173 Source E.coli-derived mousebFGF, Pro10-Ser154, with an N-terminal Met. Molecular Weight Approximately 16.5kDa. AA Sequence MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >98%by SDS-PAGEand HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 106IU/mg.Fully biologically active when compared to standard. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution inPBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.