Recombinant Rat IFN-gamma Protein

Description :
FAQ COA 已发表文献 Interferon-gamma(IFN-), also known as Type II interferon or immune interferon,is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN- exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90%amino acid (aa) sequence identity with rhesus IFN-, 59%_x005f_x001e_64%with bovine, canine, equine, feline, and porcine IFN-, and 37%_x005f_x001e_43%with cotton rat, mouse, and rat IFN-.Interferons have had increasing clinical usage in the treatment of a variety of disorders, at times being used in combination with chemo or radiotherapy.IFN- increases the potential immunogenicity of vascular endothelial cells by up-regulation of intercellular adhesion molecule-1 (ICAM-1) and class I MHC antigen expression and by induction of class II MHC antigens and certain chemokines. Product Properties Synonyms Interferon-gamma,IFN-,IFG,IFI,IFNG,IFNgamma,IFN-gamma. Accession P01581 GeneID 25712 Source E.coli-derived rat IFN- protein, Gln23-Cys156. Molecular Weight Approximately 15.5 kDa. AA Sequence QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 107IU/mg.Fully biologically active when compared to standard. Endotoxin 0.1EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 1 PBS, pH 7.4, 1 mM DTT, 5%Trehalose and 0.05%Tween-80. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.