Recombinant Rat IL-2 Protein

Description :
FAQ COA 已发表文献 Interleukin-2(IL-2)is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T- cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists ofthree subunits (55 kDa IL-2R, 75 kDa IL-2R, and 64 kDa common gamma chain c/IL-2R) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56%and 66%amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. Product Properties Synonyms Interleukin-2,IL-2,T-cell Growth Factor, TCGF, Aldesleukin Accession P17108 GeneID 116562 Source E.coli-derived ratIL-2 protein, Ala21-Gln155. Molecular Weight Approximately 15.5 kDa. AA Sequence APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 106IU/mg.Fully biologically active when compared to standard. Endotoxin EU per 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in 10 mM sodium citrate, pH 4.0. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.