Recombinant Rat IL-1 beta Protein

Description :
FAQ COA 已发表文献 Interleukin-1 beta (IL-1) is a pro inflammatory cytokine, which is secreted by immune cells to trigger inflammation and this has been found profoundly in the lesions caused by Leishmania pathogens.IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both IL-1 and IL-1 binds to the same receptor and has similar but not identical biological properties.The mature human IL1 shares 96%amino acid sequence identity with rhesus and 67%-78%with canine, mouse and rat IL-1.IL-1, are known to modulate effects of neurotoxic neurotransmitters discharged during excitation or inflammation in the central nervous system (CNS). Product Properties Synonyms Interleukin-1 beta,IL-1,IL-1,IL1B,IL-1b,IL1-BETA,IL-1F2,IL1F2IL-1 beta,interleukin-1 beta,preinterleukin 1 beta,pro-interleukin-1-beta. Accession Q63264 Unigene Rn.9869. Source E.coli-derived rat IL-1 protein, Val117-Ser268, with an N-terminal Met. Molecular Weight Approximately 17.3 kDa. AA Sequence MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 107IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4, with 5%trehalose, 0.02%Tween-20. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.