Recombinant Human BMP-4 Protein

Description :
FAQ COA 已发表文献 Bone morphogenetic protein-4 (BMP-4) is one of nine structurally related BMPs belonging to the transforming growth factor-beta (TGF-beta) superfamily of secreted proteins. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4.Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity.BMP-4, a stimulator of chondrogenesis, both in vitro and in vivo, is a potential therapeutic agent for cartilage regeneration. Product Properties Synonyms BMP2B,BMP-2B,BMP2B1, BMP2BMCOPS6,BMP4,DVR4,MCOPS6,OFC11,ZYME Accession P12644 Source E.coli-derived humanBMP-4 protein, Ser293-Arg408, E399D. Molecular Weight The recombinant Human BMP4 predicts a molecular mass of 13.25 kDa. Tag None Purity > 95% by SDS-PAGE Biological Activity Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50for this effect is typically 0.08-0.4ug/mL. Endotoxin EU per 1g of the protein by the LAL method. Formulation Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA.Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.