Recombinant Human GM-CSF Protein

Description :
FAQ COA 已发表文献 Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a powerful growth and differentiation factor which acts on hematopoietic progenitor cells and also activates differentiated granulocytes and macrophages.The 22kDa glycosylated GM_x005f_x001e_CSF, similar to IL_x005f_x001e_3 and IL_x005f_x001e_5, is a cytokine with a core of four bundledalpha -helices. Mature human GM_x005f_x001e_CSF shares 63%_x005f_x001e_70% amino acid sequence identity with canine, feline, porcine, and rat GM_x005f_x001e_CSF and 54%with mouse GM_x005f_x001e_CSF.GM-CSF has been and is still widely used as an adjuvant in clinical trials of vaccination with autologous tumor cells, peptides and/or dendritic cells in a variety of human neoplasms.The effects of GM-CSF on HIV-1 replication in primary monocytes and macrophages, and in related cell lines, have demonstrated augmentation of HIV-1 expression in vitro. Product Properties Synonyms Granulocyte/Macrophage Colony-Stimulating Factor,GM-CSF,CSF-2, MGI-1GM, Pluripoietin- Accession P04141 GeneID 1437 Source E.coli-derived human GM-CSFprotein, Ala18-Glu144. Molecular Weight Approximately 14.5kDa. AA Sequence APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98%by SDS-PAGE and HPLC analyses. Biological Activity The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 107IU/mg.Fully biologically active when compared to standard. Endotoxin EUper 1g of the protein by the LAL method. Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. Further dilutions should be made in appropriate buffered solutions.