Recombinant Mouse CD40 Ligand/TNFSF5 Protein,His Tag

Description :
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand Source Mouse CD40 Ligand/TNFSF5 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Met112-Leu260.[Accession | P27548] Molecular Weight The protein has a predicted MW of 17.50 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human CD40, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Mouse CD40 Ligand, His Tag with the EC50 of 6.9ng/ml determined by ELISA . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.