Recombinant Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein,His-Avi Tag

Description :
Synonyms V5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V 5; 5 Source Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail&Gly24-Asn719(ITGB5) basic tail.[Accession | P06756-1(ITGAV)&P18084(ITGB5)] Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Anti-alpha V Antibody, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB5, His Tag with the EC50 of 0.29ug/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.