Recombinant Cynomolgus CA9/Carbonic Anhydrase IX Protein,His Tag

Description :
Synonyms CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC Source Cynomolgus CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln38-Asp402.[Accession | A0A2K5VQG9] Molecular Weight The protein has a predicted MW of 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus CA9, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.