Recombinant Biotinylated Human FGL2 Protein,His-Avi and Flag Tag

Description :
Synonyms Fibroleukin; pT49; FGL2; fibrinogen-like 2; Source Biotinylated Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus. It contains Val205-Pro439.[Accession | Q14314] Molecular Weight The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human FGL2, His Tag at 2g/ml (100l/Well) on streptavidin (5g/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.