Recombinant Biotinylated Human DLL3 Domain (311-479) Protein,His-Avi Tag

Description :
Synonyms Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 Source Biotinylated Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus. It contains Val311-Ala479.[Accession | Q9NYJ7-1] Molecular Weight The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human DLL3 Domain (311-479) ,His Tag at 0.5g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.