Recombinant Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein,His-Avi Tag

Description :
Synonyms HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC-G; sHLA-G Source Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.[Accession | E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL] Molecular Weight The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 0.13 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.