Recombinant Biotinylated Human IL-10 R alpha Protein,His-Avi Tag

Description :
Synonyms IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha Source Biotinylated Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His22-Asn235.[Accession | Q13651] Molecular Weight The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-10, No Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.