Recombinant Human MSLN/Mesothelin Protein,His-Avi Tag

Description :
Synonyms Mesothelin;MPFSMRP;CAK1;MSLN Source Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2] Molecular Weight The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human MSLN, His Tag at 0.5g/ml (100l/Well). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.