Recombinant Biotinylated Human Peptide Ready HLA-A*03:01&B2M MonomerProtein,His-Avi Tag

Description :
Synonyms MHC; HLA-A*03:01; Peptide Ready Source Biotinylated Human Peptide Ready HLA-A*03:01&B2M MonomerProtein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)] Molecular Weight The protein has a predicted MW of 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5g/ml (100l/well) on the streptavidin precoated plate(5g/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. Formulation Supplied as 0.22m filtered solution in PBS (pH 7.4).