Recombinant Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein,His-Avi Tag

Description :
Synonyms OIP4;OIP-4;PRAME Source Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide.[Accession | P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL] Molecular Weight The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Antibody, hFc Tag with the EC50 of 40.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.