Recombinant Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein,His-Avi Tag

Description :
Synonyms IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 ; IL23 alpha&IL12 Source Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta).[Accession | Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)] Molecular Weight The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 28.1ng/ml determined by ELISA. Formulation Supplied as 0.22 m filtered solution in PBS (pH 7.4).