Recombinant Hamster FGF21Protein,His Tag

Description :
Synonyms UNQ3115; PRO10196; FGF-21; FGF21 Source Hamster FGF21Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg29-Ser209.[Accession | XP_005084765.1] Molecular Weight The protein has a predicted MW of 20.80 kDa. Due to glycosylation, the protein migrates to 21-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE,> 90% as determined by HPLC Activity SPR Data:Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.