Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein (Primary Amine Labeling) ,His-Flag Tag

Description :
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik Source Biotinylated Human APRIL/TNFSF13 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Lys112-Leu250.[Accession | O75888-1] Molecular Weight The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human BCMA, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 5.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.