Recombinant Human Latent TGF beta 1/TGFB1 Protein,His Tag

Description :
Synonyms TGF beta1;TGFB1;DPD1;CEDLAP Source Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu30-Ser390(C33S).[Accession | P01137] Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa(LAP) / 13-15 kDa(Mature TGF Beta 1) kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human Latent TGF beta 1, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA.ELISA Data: Immobilized Human Latent TGF beta 1, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 6.6ug/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.