Recombinant Cynomolgus RGM-C Protein,His Tag

Description :
Synonyms Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C Source Cynomolgus RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln36-Asp401.[Accession | XP_005542141.1] Molecular Weight The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa&30-40 kDa&20-25 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus RGM-C, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.