Recombinant Biotinylated Cynomolgus/Rhesus macaque FcRn Protein,His-Avi Tag

Description :
Synonyms FcRn alpha chain; FCGRT; FcRn; FCGRT&B2M Source Biotinylated Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala24-Ser297(FCGRT) and Ile21-Met119(B2M).[Accession | Q8SPV9&Q8SPW0] Molecular Weight The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Biotinylated Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.48 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.