Recombinant Biotinylated Human Peptide Ready HLA-A*02:01&B2M MonomerProtein,His-Avi Tag

Description :
Synonyms MHC; HLA-A*02:01; Peptide Ready Source Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M).[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)] Molecular Weight The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.