Recombinant Mouse TRAIL R2/DR5/TNFRSF10B Protein,His Tag

Description :
Synonyms CD262; Fas-like protein; DR5TRICK2B; TNFRSF10B Source Mouse TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asn53-Lys180.[Accession | Q9QZM4-1] Molecular Weight The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Mouse TRAIL R2, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.43 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.