Recombinant Human TNFRSF11B Protein,His Tag

Description :
Synonyms Opg; Ocif;Osteoprotegerin; TR1 Source Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu22-Leu401.[Accession | O00300] Molecular Weight The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human RANKL, No Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.