Recombinant Human DKK1 C terminal Domain Protein,hFc-Avi Tag

Description :
Synonyms Dkk-1; Dickkopf-1;Dickkopf-related 1; hDkk-1 Source Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Met178-His266.[Accession | O94907] Molecular Weight The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).