Recombinant Biotinylated Human IL-22R alpha 1&IL-10R beta Protein,hFc-Avi Tag

Description :
Synonyms IL-22R1&IL-10R; IL-10R beta;IL-22R alpha 1; Source Biotinylated Human IL-22R alpha 1&IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus. It contains His16-Thr228(IL-22R alpha 1)&Met20-Ser220(IL-10R beta).[Accession | Q8N6P7(IL-22R alpha 1)&Q08334(IL-10R beta)] Molecular Weight The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-22, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-22R alpha1&IL-10R beta, hFc Tag with the EC50 of 0.63g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.