Recombinant Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein,His-Avi Tag

Description :
Synonyms TGAV&ITGB8; Integrin alpha V beta 8;V8; Source Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail.[Accession | P06756-1(ITGAV)&P26012-1(ITGB8)] Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&76 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-150 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAV&ITGB8, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.