Recombinant Cynomolgus B7-1/CD80 Protein,His Tag

Description :
Synonyms CD80 molecule; B7-1; B71; CD28LG1 Source Cynomolgus B7-1/CD80 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val35-Asn242.[Accession | G7NXN7] Molecular Weight The protein has a predicted MW of 25 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.