Recombinant Human Siglec-4a/MAG Protein,hFc Tag

Description :
Synonyms Siglec-4a; Siglec4a;MAG; GMA Source Human Siglec-4a/MAG Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly20-Pro516.[Accession | P20916] Molecular Weight The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Siglec-4a, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.