Recombinant Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein,His Tag

Description :
Synonyms ITGAV&ITGB8; Integrin alpha V beta 8;V8; Source Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe31-Val988(ITGAV) acidic tail&Gly22-Ser679(ITGB8) basic tail.[Accession | P43406(ITGAV)&Q0VBD0(ITGB8)] Molecular Weight The protein has a predicted MW of 111.6(ITGAV)&76.6(ITGB8) kDa. Due to glycosylation, the protein migrates to 80-140 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse ITGAV&ITGB8, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 2.4g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.