Recombinant Human NGAL/Lipocalin-2 Protein,His Tag

Description :
Synonyms Oncogene 24p3; Lipocalin-2;NGAL; Siderocalin LCN2 Source Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln21-Gly198.[Accession | P80188-1] Molecular Weight The protein has a predicted MW of 21.64 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human NGAL, His Tag at 0.2g/ml (100l/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.