Recombinant Biotinylated Human B7-H6 Protein,His-Avi Tag

Description :
Synonyms B7H6; DKFZp686O24166; DKFZp686I21167; B7 Homolog 6 Source Biotinylated Human B7-H6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp25-Ser262.[Accession | Q68D85] Molecular Weight The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.