Recombinant Biotinylated Mouse IL-17A&F Protein,His-Avi Tag

Description :
Synonyms CTLA-8;Interleukin-17A;IL-17A;IL-17F Source Biotinylated Mouse IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F).[Accession | Q62386(IL-17A)&Q7TNI7-1(IL-17F)] Molecular Weight The protein has a predicted MW of 17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse IL-17R alpha, hFc Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.