Recombinant Cynomolgus B7-H2/ICOSLG Protein,His Tag

Description :
Synonyms CD275; B7RP-1; B7-H2; ICOSL Source Cynomolgus B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp19-Thr256.[Accession | XP_005548617.1] Molecular Weight The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.