Recombinant Human Coagulation Factor III/Tissue Factor Protein,His Tag

Description :
Synonyms TF; Coagulation Factor III;Tissue factor; CD142 Source Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser33-Glu251.[Accession | P13726-1] Molecular Weight The protein has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Coagulation Factor III, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.