Recombinant Human IL-6 R alpha/CD126 Protein,hFc Tag

Description :
Synonyms IL-6RA; IL-6R-alpha;IL-6R subunit alpha; IL6R Source Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu20-Pro365.[Accession | P08887-1] Molecular Weight The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human IL-6 at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.