Recombinant Cynomolgus GDF15 Protein,hFc Tag

Description :
Synonyms NAG-1; MIC-1;GDF-15; PLAB Source Cynomolgus GDF15 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala197-Val308.[Accession | G7PWZ3] Molecular Weight The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Cynomolgus GFRAL, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Cynomolgus GDF15, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.